Transposon signatures: species‐specific molecular markers that utilize a class of multiple‐copy nuclear DNA

M. D. Purugganan, S. R. Wessler

Research output: Contribution to journalArticle

Abstract

Transposable elements are mobile sequences found in nuclear genomes and can potentially serve as molecular markers in various phylogenetic and population genetic investigations. A PCR‐based method that utilizes restriction site variation of element copies within a genome is developed. These patterns of site variation, referred to as transposon signatures, are useful in differentiating between closely related groups. Signature data using the magellan retrotransposon, for example, is useful in examining relationships within the genus Zea and Tripsacum. This method allows transposable elements, or even other multiple‐copy nuclear DNA sequences, to be generally utilized as molecular markers in discriminating between other closely related species and subspecies.

Original languageEnglish (US)
Pages (from-to)265-270
Number of pages6
JournalMolecular ecology
Volume4
Issue number2
DOIs
StatePublished - Apr 1995

Keywords

  • DNA fingerprinting
  • Tripsacum
  • Zea
  • restriction site polymorphism
  • retrotransposon

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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