Trna genes rapidly change in evolution to meet novel translational demands

Avihu H. Yona, Zohar Bloom-Ackermann, Idan Frumkin, Victor Hanson-Smith, Yoav Charpak-Amikam, Qinghua Feng, Jef D. Boeke, Orna Dahan, Yitzhak Pilpel

    Research output: Contribution to journalArticle

    Abstract

    Changes in expression patterns may occur when organisms are presented with new environmental challenges, for example following migration or genetic changes. To elucidate the mechanisms by which the translational machinery adapts to such changes, we perturbed the tRNA pool of Saccharomyces cerevisiae by tRNA gene deletion. We then evolved the deletion strain and observed that the genetic adaptation was recurrently based on a strategic mutation that changed the anticodon of other tRNA genes to match that of the deleted one. Strikingly, a systematic search in hundreds of genomes revealed that anticodon mutations occur throughout the tree of life. We further show that the evolution of the tRNA pool also depends on the need to properly couple translation to protein folding. Together, our observations shed light on the evolution of the tRNA pool, demonstrating that mutation in the anticodons of tRNA genes is a common adaptive mechanism when meeting new translational demands.

    Original languageEnglish (US)
    Article numbere01339
    JournaleLife
    Volume2013
    Issue number2
    DOIs
    StatePublished - Dec 20 2013

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Immunology and Microbiology(all)
    • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Yona, A. H., Bloom-Ackermann, Z., Frumkin, I., Hanson-Smith, V., Charpak-Amikam, Y., Feng, Q., Boeke, J. D., Dahan, O., & Pilpel, Y. (2013). Trna genes rapidly change in evolution to meet novel translational demands. eLife, 2013(2), [e01339]. https://doi.org/10.7554/eLife.01339