Trophic interactions between brain-derived neurotrophic factor and S100β on cultured serotonergic neurons

Mayumi Nishi; Mitsuhiro Kawata; Efrain C. Azmitia

doi:10.1016/S0006-8993(00)02201-0

Trophic interactions between brain-derived neurotrophic factor and S100β on cultured serotonergic neurons

Mayumi Nishi, Mitsuhiro Kawata, Efrain C. Azmitia

Biology and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

Brain-derived neurotrophic factor (BDNF) and S100β stimulate serotonergic neurons in fetal rat raphe primary cultures grown under serum- free conditions. BDNF (50 ng/ml) treatment for 3 h enhanced S100β immunoreactivity in both raphe and hippocampal glial cells. Combined treatment with BDNF and S100β for 3 days increased the soma area of 5-HT neurons, but not the neurite length. Our results suggest that BDNF and S100β, which regulate different signal transduction cascades, interact to exert complimentary effects on neuronal maturation by acting sequentially, not concurrently. (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	113-118
Number of pages	6
Journal	Brain Research
Volume	868
Issue number	1
DOIs	https://doi.org/10.1016/S0006-8993(00)02201-0
State	Published - Jun 16 2000

Keywords

BDNF
MAP kinase
Neuronal-glial interaction
Raphe
Tissue culture

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(00)02201-0

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title = "Trophic interactions between brain-derived neurotrophic factor and S100β on cultured serotonergic neurons",

abstract = "Brain-derived neurotrophic factor (BDNF) and S100β stimulate serotonergic neurons in fetal rat raphe primary cultures grown under serum- free conditions. BDNF (50 ng/ml) treatment for 3 h enhanced S100β immunoreactivity in both raphe and hippocampal glial cells. Combined treatment with BDNF and S100β for 3 days increased the soma area of 5-HT neurons, but not the neurite length. Our results suggest that BDNF and S100β, which regulate different signal transduction cascades, interact to exert complimentary effects on neuronal maturation by acting sequentially, not concurrently. (C) 2000 Elsevier Science B.V.",

keywords = "BDNF, MAP kinase, Neuronal-glial interaction, Raphe, Tissue culture",

author = "Mayumi Nishi and Mitsuhiro Kawata and Azmitia, {Efrain C.}",

note = "Funding Information: We are grateful to Dr. L. Mamounas for helpful discussions during the course of this work. We would like to thank Xia Ping Hou for excellent technical assistance. This work was supported by funds from MH55250 to ECA and Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture, Japan, to MN and MK.",

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doi = "10.1016/S0006-8993(00)02201-0",

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AU - Nishi, Mayumi

AU - Kawata, Mitsuhiro

AU - Azmitia, Efrain C.

N1 - Funding Information: We are grateful to Dr. L. Mamounas for helpful discussions during the course of this work. We would like to thank Xia Ping Hou for excellent technical assistance. This work was supported by funds from MH55250 to ECA and Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture, Japan, to MN and MK.

PY - 2000/6/16

Y1 - 2000/6/16

N2 - Brain-derived neurotrophic factor (BDNF) and S100β stimulate serotonergic neurons in fetal rat raphe primary cultures grown under serum- free conditions. BDNF (50 ng/ml) treatment for 3 h enhanced S100β immunoreactivity in both raphe and hippocampal glial cells. Combined treatment with BDNF and S100β for 3 days increased the soma area of 5-HT neurons, but not the neurite length. Our results suggest that BDNF and S100β, which regulate different signal transduction cascades, interact to exert complimentary effects on neuronal maturation by acting sequentially, not concurrently. (C) 2000 Elsevier Science B.V.

AB - Brain-derived neurotrophic factor (BDNF) and S100β stimulate serotonergic neurons in fetal rat raphe primary cultures grown under serum- free conditions. BDNF (50 ng/ml) treatment for 3 h enhanced S100β immunoreactivity in both raphe and hippocampal glial cells. Combined treatment with BDNF and S100β for 3 days increased the soma area of 5-HT neurons, but not the neurite length. Our results suggest that BDNF and S100β, which regulate different signal transduction cascades, interact to exert complimentary effects on neuronal maturation by acting sequentially, not concurrently. (C) 2000 Elsevier Science B.V.

KW - BDNF

KW - MAP kinase

KW - Neuronal-glial interaction

KW - Raphe

KW - Tissue culture

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Trophic interactions between brain-derived neurotrophic factor and S100β on cultured serotonergic neurons

Abstract

Keywords

ASJC Scopus subject areas

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