TY - JOUR
T1 - Twice-daily red and blue light treatment for Candida albicans biofilm matrix development control
AU - da Silveira, Paula Ventura
AU - Panariello, Beatriz Helena Dias
AU - de Araújo Costa, Cecília Atem Gonçalves
AU - Maule, Shawn M.
AU - Maule, Shane M.
AU - Janal, Malvin N.
AU - Zanin, Iriana Carla Junqueira
AU - Duarte, Simone
N1 - Funding Information:
We thank Dr. Alexander D. Johnson, Department of Microbiology and Immunology, UCSF, for his generous donation of the strain used in this study.
Funding Information:
Funding information This research was supported by CAPES Foundation from whom the first author received a scholarship (CAPES 88881.062159 2014-01 PVE CAPES). The funder had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
PY - 2019/4/4
Y1 - 2019/4/4
N2 - Phototherapy has been proposed as a direct means of affecting local bacterial infections. However, the use of phototherapy to prevent fungal biofilm development has received comparatively less attention. This study aimed to determine the effects of red light treatment and blue light treatment, without a photosensitizer, on the development of Candida albicans biofilm. During the development of 48-h biofilms of C. albicans SN 425 (n = 10), the biofilms were exposed twice-daily to noncoherent blue and red light (LumaCare; 420 nm and 635 nm). The energy density applied was 72 J cm −2 for blue light and 43.8 J cm 2 , 87.6 J cm 2 , and 175.5 J cm 2 for red light. Positive control (PC) and negative control (NC) groups were treated twice-daily for 1 min with 0.12% chlorhexidine (CHX) and 0.89% NaCl respectively. Biofilms were analyzed for colony forming units (CFU), dry-weight, and exopolysaccharides (EPS-soluble and EPS-insoluble). Data was analyzed by one-way ANOVA and Tukey post hoc test (α = 0.05). Dry-weight was lower than NC (p < 0.001) and approached PC levels with both red and blue light treatments. CFU were also lower in groups exposed to blue light and higher durations of red light (p < 0.05). EPS-soluble and EPS-insoluble measures were variably reduced by these light exposures. In conclusion, twice-daily exposure to both blue and red lights affect the biofilm development and physiology of polysaccharide production and are potential mechanisms for the control of C. albicans biofilm matrix development.
AB - Phototherapy has been proposed as a direct means of affecting local bacterial infections. However, the use of phototherapy to prevent fungal biofilm development has received comparatively less attention. This study aimed to determine the effects of red light treatment and blue light treatment, without a photosensitizer, on the development of Candida albicans biofilm. During the development of 48-h biofilms of C. albicans SN 425 (n = 10), the biofilms were exposed twice-daily to noncoherent blue and red light (LumaCare; 420 nm and 635 nm). The energy density applied was 72 J cm −2 for blue light and 43.8 J cm 2 , 87.6 J cm 2 , and 175.5 J cm 2 for red light. Positive control (PC) and negative control (NC) groups were treated twice-daily for 1 min with 0.12% chlorhexidine (CHX) and 0.89% NaCl respectively. Biofilms were analyzed for colony forming units (CFU), dry-weight, and exopolysaccharides (EPS-soluble and EPS-insoluble). Data was analyzed by one-way ANOVA and Tukey post hoc test (α = 0.05). Dry-weight was lower than NC (p < 0.001) and approached PC levels with both red and blue light treatments. CFU were also lower in groups exposed to blue light and higher durations of red light (p < 0.05). EPS-soluble and EPS-insoluble measures were variably reduced by these light exposures. In conclusion, twice-daily exposure to both blue and red lights affect the biofilm development and physiology of polysaccharide production and are potential mechanisms for the control of C. albicans biofilm matrix development.
KW - Biofilm
KW - Blue light
KW - Candida albicans
KW - Phototherapy
KW - Red light
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U2 - 10.1007/s10103-018-2610-x
DO - 10.1007/s10103-018-2610-x
M3 - Article
C2 - 30097756
AN - SCOPUS:85051301948
SN - 0268-8921
VL - 34
SP - 441
EP - 447
JO - Lasers in Medical Science
JF - Lasers in Medical Science
IS - 3
ER -