Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila

Alexis Gambis; Pierre Dourlen; Hermann Steller; Bertrand Mollereau

doi:10.1016/j.ydbio.2010.12.040

Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila

Alexis Gambis, Pierre Dourlen, Hermann Steller, Bertrand Mollereau

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

We report a new two-color fluorescent imaging system to visualize the mosaic adult photoreceptor neurons (PRs) in real-time. Using this method, we examined a collection of 434 mutants and identified genes required for PR survival, planar cell polarity (PCP), patterning and differentiation. We could track the progression of PR degeneration in living flies. By introducing the expression of p35, a caspase inhibitor, we found mutations that specifically activate caspase-dependent death. Moreover, we showed that grh is required in R3 for correct PCP establishment. The "Tomato/GFP-FLP/FRT" method allows high-throughput, rapid and precise identification of survival and developmental pathways in living adult PRs at single-cell resolution.

Original language	English (US)
Pages (from-to)	128-134
Number of pages	7
Journal	Developmental Biology
Volume	351
Issue number	1
DOIs	https://doi.org/10.1016/j.ydbio.2010.12.040
State	Published - Mar 1 2011

Keywords

Apoptosis
Development
Neuron
Polarity
Retina

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

Access to Document

10.1016/j.ydbio.2010.12.040

Cite this

@article{752ebe980d4146ec88274f8c88604682,

title = "Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila",

abstract = "We report a new two-color fluorescent imaging system to visualize the mosaic adult photoreceptor neurons (PRs) in real-time. Using this method, we examined a collection of 434 mutants and identified genes required for PR survival, planar cell polarity (PCP), patterning and differentiation. We could track the progression of PR degeneration in living flies. By introducing the expression of p35, a caspase inhibitor, we found mutations that specifically activate caspase-dependent death. Moreover, we showed that grh is required in R3 for correct PCP establishment. The {"}Tomato/GFP-FLP/FRT{"} method allows high-throughput, rapid and precise identification of survival and developmental pathways in living adult PRs at single-cell resolution.",

keywords = "Apoptosis, Development, Neuron, Polarity, Retina",

author = "Alexis Gambis and Pierre Dourlen and Hermann Steller and Bertrand Mollereau",

note = "Funding Information: This work was supported by grants from the National Institute of Health ( ROI EY14025 ), from the Fondation pour la Recherche M{\'e}dicale , from the CNRS (ATIP) to B.M. H.S. is an investigator of the Howard Hughes Medical Institute and part of this work was supported by NIH grant RO1GM60124 to H.S. A.G. is a former student at the David Rockefeller Graduate Program at the Rockefeller University. P.D. is supported by Retina France association . This work was made possible by the DROSO-TOOLS and PLATIM facilities of the IFR128, Biosciences, Lyon, France. We thank Dali Ma for critical reading of the manuscript and our colleagues and Bloomington center for fly stocks and reagents.",

year = "2011",

month = mar,

day = "1",

doi = "10.1016/j.ydbio.2010.12.040",

language = "English (US)",

volume = "351",

pages = "128--134",

journal = "Developmental Biology",

issn = "0012-1606",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila

AU - Gambis, Alexis

AU - Dourlen, Pierre

AU - Steller, Hermann

AU - Mollereau, Bertrand

N1 - Funding Information: This work was supported by grants from the National Institute of Health ( ROI EY14025 ), from the Fondation pour la Recherche Médicale , from the CNRS (ATIP) to B.M. H.S. is an investigator of the Howard Hughes Medical Institute and part of this work was supported by NIH grant RO1GM60124 to H.S. A.G. is a former student at the David Rockefeller Graduate Program at the Rockefeller University. P.D. is supported by Retina France association . This work was made possible by the DROSO-TOOLS and PLATIM facilities of the IFR128, Biosciences, Lyon, France. We thank Dali Ma for critical reading of the manuscript and our colleagues and Bloomington center for fly stocks and reagents.

PY - 2011/3/1

Y1 - 2011/3/1

N2 - We report a new two-color fluorescent imaging system to visualize the mosaic adult photoreceptor neurons (PRs) in real-time. Using this method, we examined a collection of 434 mutants and identified genes required for PR survival, planar cell polarity (PCP), patterning and differentiation. We could track the progression of PR degeneration in living flies. By introducing the expression of p35, a caspase inhibitor, we found mutations that specifically activate caspase-dependent death. Moreover, we showed that grh is required in R3 for correct PCP establishment. The "Tomato/GFP-FLP/FRT" method allows high-throughput, rapid and precise identification of survival and developmental pathways in living adult PRs at single-cell resolution.

AB - We report a new two-color fluorescent imaging system to visualize the mosaic adult photoreceptor neurons (PRs) in real-time. Using this method, we examined a collection of 434 mutants and identified genes required for PR survival, planar cell polarity (PCP), patterning and differentiation. We could track the progression of PR degeneration in living flies. By introducing the expression of p35, a caspase inhibitor, we found mutations that specifically activate caspase-dependent death. Moreover, we showed that grh is required in R3 for correct PCP establishment. The "Tomato/GFP-FLP/FRT" method allows high-throughput, rapid and precise identification of survival and developmental pathways in living adult PRs at single-cell resolution.

KW - Apoptosis

KW - Development

KW - Neuron

KW - Polarity

KW - Retina

UR - http://www.scopus.com/inward/record.url?scp=79551682600&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79551682600&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2010.12.040

DO - 10.1016/j.ydbio.2010.12.040

M3 - Article

C2 - 21215264

AN - SCOPUS:79551682600

SN - 0012-1606

VL - 351

SP - 128

EP - 134

JO - Developmental Biology

JF - Developmental Biology

IS - 1

ER -

Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this