Two-Photon, Ratiometric, Quantitative Fluorescent Probe Reveals Fluctuation of Peroxynitrite Regulated by Arginase 1

Shiyu Chen, Beyza Vurusaner, Stephanie Pena, Chu T. Thu, Lara Mahal, Edward A. Fisher, James W. Canary

Research output: Contribution to journalArticlepeer-review

Abstract

Peroxynitrite, a transient reactive oxygen species (ROS), is believed to play a deleterious role in physiological processes. Herein, we report a two-photon ratiometric fluorescent probe that selectively reacts with peroxynitrite yielding a >200-fold change upon reaction. The probe effectively visualized fluctuations in peroxynitrite generation by arginase 1 in vivo and in vitro. This provides evidence that arginase 1 is a critical regulator of peroxynitrite.

Original languageEnglish (US)
Pages (from-to)10090-10098
Number of pages9
JournalAnalytical Chemistry
Volume93
Issue number29
DOIs
StatePublished - Jul 27 2021

ASJC Scopus subject areas

  • Analytical Chemistry

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