Type x collagen synthesis in human osteoarthritic cartilage. indication of chondrocyte hypertrophy

K. Von Der Mark, T. Kirsch, A. Nerlich, A. Kuss, G. Weseloh, K. Glückert, H. Stöss

    Research output: Contribution to journalArticle

    Abstract

    Objective. To investigate the appearance of hypertrophic chondrocytes in osteoarthritic (OA) cartilage, using type X collagen as a specific marker. Methods. The biosynthesis of type X collagen was examined by metabolic labeling of freshly isolated articular chondrocytes with 3H‐proline, immunoprecipitation, and sodium dodecyl sulfate–polyacrylamide gel electrophoresis of the synthesized collagens. Extracellular deposition of types X and II collagen was analyzed immunohistochemically. Results. Immunostaining revealed an irregular distribution of type X collagen, which was localized around chondrocyte clusters in fibrillated OA cartilage, but was absent from the noncalcified region of normal articular cartilage. Freshly isolated OA chondrocytes synthesized predominantly type X collagen, while control chondrocytes synthesized mostly type II collagen. Conclusion. Our findings indicate focal premature chondrocyte differentiation to hypertrophic cells in OA cartilage.

    Original languageEnglish (US)
    Pages (from-to)806-811
    Number of pages6
    JournalArthritis and Rheumatism
    Volume35
    Issue number7
    DOIs
    StatePublished - Jul 1992

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Rheumatology
    • Immunology
    • Pharmacology (medical)

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    Von Der Mark, K., Kirsch, T., Nerlich, A., Kuss, A., Weseloh, G., Glückert, K., & Stöss, H. (1992). Type x collagen synthesis in human osteoarthritic cartilage. indication of chondrocyte hypertrophy. Arthritis and Rheumatism, 35(7), 806-811. https://doi.org/10.1002/art.1780350715