Untargeted LC-MS Metabolomics for the Analysis of Micro-scaled Extracellular Metabolites from Hepatocytes

Rodi Abdalkader; Romanas Chaleckis; Isabel Meister; Pei Zhang; Craig E. Wheelock; Ken Ichiro Kamei

doi:10.2116/analsci.20N032

Untargeted LC-MS Metabolomics for the Analysis of Micro-scaled Extracellular Metabolites from Hepatocytes

Rodi Abdalkader, Romanas Chaleckis, Isabel Meister, Pei Zhang, Craig E. Wheelock, Ken Ichiro Kamei

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Metabolome analysis in micro physiological models is a challenge due to the low volume of the cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS method we have detected 57 metabolites of which 27 showed >2-fold shifts after 72-hour incubation. We demonstrate that micro-scale CCM samples can be used for modelling micro-physiological temporal dynamics in metabolite intensities.

Original language	English (US)
Pages (from-to)	1049-1052
Number of pages	4
Journal	Analytical Sciences
Volume	37
Issue number	7
DOIs	https://doi.org/10.2116/analsci.20N032
State	Published - 2021

Keywords

Hepatocyte
LC-MS
extracellular metabolites

ASJC Scopus subject areas

Analytical Chemistry

Access to Document

10.2116/analsci.20N032

Cite this

@article{a6454c51e133444cb39f340e4292a4c1,

title = "Untargeted LC-MS Metabolomics for the Analysis of Micro-scaled Extracellular Metabolites from Hepatocytes",

abstract = "Metabolome analysis in micro physiological models is a challenge due to the low volume of the cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS method we have detected 57 metabolites of which 27 showed >2-fold shifts after 72-hour incubation. We demonstrate that micro-scale CCM samples can be used for modelling micro-physiological temporal dynamics in metabolite intensities.",

keywords = "Hepatocyte, LC-MS, extracellular metabolites",

author = "Rodi Abdalkader and Romanas Chaleckis and Isabel Meister and Pei Zhang and Wheelock, {Craig E.} and Kamei, {Ken Ichiro}",

note = "Publisher Copyright: 2021 {\textcopyright} The Japan Society for Analytical Chemistry",

year = "2021",

doi = "10.2116/analsci.20N032",

language = "English (US)",

volume = "37",

pages = "1049--1052",

journal = "Analytical Sciences",

issn = "0910-6340",

publisher = "Springer International Publishing AG",

number = "7",

}

TY - JOUR

T1 - Untargeted LC-MS Metabolomics for the Analysis of Micro-scaled Extracellular Metabolites from Hepatocytes

AU - Abdalkader, Rodi

AU - Chaleckis, Romanas

AU - Meister, Isabel

AU - Zhang, Pei

AU - Wheelock, Craig E.

AU - Kamei, Ken Ichiro

N1 - Publisher Copyright: 2021 © The Japan Society for Analytical Chemistry

PY - 2021

Y1 - 2021

N2 - Metabolome analysis in micro physiological models is a challenge due to the low volume of the cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS method we have detected 57 metabolites of which 27 showed >2-fold shifts after 72-hour incubation. We demonstrate that micro-scale CCM samples can be used for modelling micro-physiological temporal dynamics in metabolite intensities.

AB - Metabolome analysis in micro physiological models is a challenge due to the low volume of the cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS method we have detected 57 metabolites of which 27 showed >2-fold shifts after 72-hour incubation. We demonstrate that micro-scale CCM samples can be used for modelling micro-physiological temporal dynamics in metabolite intensities.

KW - Hepatocyte

KW - LC-MS

KW - extracellular metabolites

UR - http://www.scopus.com/inward/record.url?scp=85107672623&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85107672623&partnerID=8YFLogxK

U2 - 10.2116/analsci.20N032

DO - 10.2116/analsci.20N032

M3 - Article

C2 - 33342928

AN - SCOPUS:85107672623

SN - 0910-6340

VL - 37

SP - 1049

EP - 1052

JO - Analytical Sciences

JF - Analytical Sciences

IS - 7

ER -

Untargeted LC-MS Metabolomics for the Analysis of Micro-scaled Extracellular Metabolites from Hepatocytes

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this