Untargeted LC-MS Metabolomics for the Analysis of Micro-scaled Extracellular Metabolites from Hepatocytes

Rodi Abdalkader, Romanas Chaleckis, Isabel Meister, Pei Zhang, Craig E. Wheelock, Ken Ichiro Kamei

Research output: Contribution to journalArticlepeer-review

Abstract

Metabolome analysis in micro physiological models is a challenge due to the low volume of the cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS method we have detected 57 metabolites of which 27 showed >2-fold shifts after 72-hour incubation. We demonstrate that micro-scale CCM samples can be used for modelling micro-physiological temporal dynamics in metabolite intensities.

Original languageEnglish (US)
Pages (from-to)1049-1052
Number of pages4
JournalAnalytical Sciences
Volume37
Issue number7
DOIs
StatePublished - 2021

Keywords

  • extracellular metabolites
  • Hepatocyte
  • LC-MS

ASJC Scopus subject areas

  • Analytical Chemistry

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