The availability of molecular genetic tools and techniques has had a profound impact on the analysis of the immune response to viral infection. This promises to result in the rapid development of vaccines and therapeutics for these important diseases. This chapter focuses on the experimental procedures that are used to analyze the humoral and cellular immune responses to a virus infection. It focuses on stomatitis virus, an RNA virus closely related to rabies. This virus has a glycoprotein that is embedded in the lipid envelope, a single strand of RNA, which is coated with a nucleoprotein, phosphoprotein and large protein, and a matrix protein. There are two principal serotypes (Indiana and New Jersey), which are principally distinguished by changes in amino acids in the glycoprotein. In the experimental model used, virus is applied to the nose of the mouse and causes an infection that enters the brain via the olfactory nerve.
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)