Viral restricted cytolytic T lymphocyte recognition of hybrid human-murine class I histocompatibility antigens

Richard T. Maziarz, Steven J. Burakoff, Carol S. Reiss

Research output: Contribution to journalArticle

Abstract

Hybrid human-murine major histocompatibility antigens have been constructed and expressed on the surface of both human RD and murine L cell lines after DNA mediated gene transfer. These antigens linked the polymorphic domains (α1 and α2) of H-2Kb and the carboxy-terminal domains (α3, transmembrane, and intracellular) of HLA-A2. Previously we demonstrated that these antigens were serologically intact and were recognized by allospecific cytolytic T lymphocytes. However, the cell lines expressing the hybrid antigen were less well lysed than the native H-2Kb expressing cell lines. In this study, we extend these observations and demonstrate that virally restricted cytolytic T lymphocytes specific for vesicular stomatitis virus and for Sendai virus can recognize cell lines expressing the hybrid antigen, whether expressed on murine (L cell) or human (RD cell) lines. Furthermore, the data show a profound influence by the carboxy-terminal domains upon the polymorphic T-cell restricting epitopes.

Original languageEnglish (US)
Pages (from-to)279-286
Number of pages8
JournalCellular Immunology
Volume99
Issue number1
DOIs
StatePublished - Apr 15 1986

ASJC Scopus subject areas

  • Immunology

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