What can we learn from a small regulatory membrane protein?

Gianluigi Veglia, Kim N. Ha, Lei Shi, Raffaello Verardi, Nathaniel J. Traaseth

Research output: Contribution to journalArticle

Abstract

This chapter reviews the molecular biology, biochemical, and NMR methods that we used to study the structural dynamics, membrane topology, and interaction of phospholamban (PLN), a small regulatory membrane protein involved in the regulation of the sarcoplasmic reticulum Ca-ATPase (SERCA). In particular, we show the progression of our research from the initial hypotheses toward understanding the molecular mechanisms of SERCA's regulation, including the effects of PLN oligomerization and posttranslational phosphorylation. Finally, we show how the knowledge of the molecular mechanism of the structural dynamics and topology of free and bound proteins can lead to the rational design of PLN analogs for possible use in gene therapy.

Original languageEnglish (US)
Pages (from-to)303-319
Number of pages17
JournalMethods in molecular biology (Clifton, N.J.)
Volume654
DOIs
StatePublished - 2010

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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