Whispering-gallery-mode biosensing: Label-free detection down to single molecules

Frank Vollmer; Stephen Arnold

doi:10.1038/nmeth.1221

Whispering-gallery-mode biosensing: Label-free detection down to single molecules

Research output: Contribution to journal › Article › peer-review

Abstract

Optical label-free detectors, such as the venerable surface plasmon resonance (SPR) sensor, are generally favored for their ability to obtain quantitative data on intermolecular binding. However, before the recent introduction of resonant microcavities that use whispering gallery mode (WGM) recirculation, sensitivity to single binding events had not materialized. Here we describe the enhancement mechanisms responsible for the extreme sensitivity of the WGM biosensor, review its current implementations and applications, and discuss its future possibilities.

Original language	English (US)
Pages (from-to)	591-596
Number of pages	6
Journal	Nature methods
Volume	5
Issue number	7
DOIs	https://doi.org/10.1038/nmeth.1221
State	Published - Jul 2008

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1038/nmeth.1221

Cite this

@article{139d1b9df0ed49c4b0245ac0e405d394,

title = "Whispering-gallery-mode biosensing: Label-free detection down to single molecules",

abstract = "Optical label-free detectors, such as the venerable surface plasmon resonance (SPR) sensor, are generally favored for their ability to obtain quantitative data on intermolecular binding. However, before the recent introduction of resonant microcavities that use whispering gallery mode (WGM) recirculation, sensitivity to single binding events had not materialized. Here we describe the enhancement mechanisms responsible for the extreme sensitivity of the WGM biosensor, review its current implementations and applications, and discuss its future possibilities.",

author = "Frank Vollmer and Stephen Arnold",

note = "Funding Information: S.A. was supported by the US National Science Foundation grant BES-0522668, and F.V. was supported by a Rowland Junior Fellowship.",

year = "2008",

month = jul,

doi = "10.1038/nmeth.1221",

language = "English (US)",

volume = "5",

pages = "591--596",

journal = "Nature methods",

issn = "1548-7091",

publisher = "Public Library of Science",

number = "7",

}

TY - JOUR

T1 - Whispering-gallery-mode biosensing

T2 - Label-free detection down to single molecules

AU - Vollmer, Frank

AU - Arnold, Stephen

N1 - Funding Information: S.A. was supported by the US National Science Foundation grant BES-0522668, and F.V. was supported by a Rowland Junior Fellowship.

PY - 2008/7

Y1 - 2008/7

N2 - Optical label-free detectors, such as the venerable surface plasmon resonance (SPR) sensor, are generally favored for their ability to obtain quantitative data on intermolecular binding. However, before the recent introduction of resonant microcavities that use whispering gallery mode (WGM) recirculation, sensitivity to single binding events had not materialized. Here we describe the enhancement mechanisms responsible for the extreme sensitivity of the WGM biosensor, review its current implementations and applications, and discuss its future possibilities.

AB - Optical label-free detectors, such as the venerable surface plasmon resonance (SPR) sensor, are generally favored for their ability to obtain quantitative data on intermolecular binding. However, before the recent introduction of resonant microcavities that use whispering gallery mode (WGM) recirculation, sensitivity to single binding events had not materialized. Here we describe the enhancement mechanisms responsible for the extreme sensitivity of the WGM biosensor, review its current implementations and applications, and discuss its future possibilities.

UR - http://www.scopus.com/inward/record.url?scp=46249089703&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=46249089703&partnerID=8YFLogxK

U2 - 10.1038/nmeth.1221

DO - 10.1038/nmeth.1221

M3 - Article

C2 - 18587317

AN - SCOPUS:46249089703

SN - 1548-7091

VL - 5

SP - 591

EP - 596

JO - Nature methods

JF - Nature methods

IS - 7

ER -

Whispering-gallery-mode biosensing: Label-free detection down to single molecules

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this