Whole-genome shotgun optical mapping of Deinococcus radiodurans

Jieyi Lin, Rong Qi, Christopher Aston, Junping Jing, Thomas S. Anantharaman, Bud Mishra, Owen White, Michael J. Daly, Kenneth W. Minton, J. Craig Venter, David C. Schwartz

Research output: Contribution to journalArticle

Abstract

A whole-genome restriction map of Deinococcus radiodurans, a radiation- resistant bacterium able to survive up to 15,000 grays of ionizing radiation, was constructed without using DNA libraries, the polymerase chain reaction, or electrophoresis. Very large, randomly sheared, genomic DNA fragments were used to construct maps from individual DNA molecules that were assembled into two circular overlapping maps (2.6 and 0.415 megabases), without gaps. A third smaller chromosome (176 kilobases)was identified and characterized. Aberrant nonlinear DNA structures that may define chromosome structure and organization, as well as intermediates in DNA repair, were directly visualized by optical mapping techniques after γ irradiation.

Original languageEnglish (US)
Pages (from-to)1558-1562
Number of pages5
JournalScience
Volume285
Issue number5433
DOIs
StatePublished - Sep 3 1999

ASJC Scopus subject areas

  • General

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    Lin, J., Qi, R., Aston, C., Jing, J., Anantharaman, T. S., Mishra, B., White, O., Daly, M. J., Minton, K. W., Venter, J. C., & Schwartz, D. C. (1999). Whole-genome shotgun optical mapping of Deinococcus radiodurans. Science, 285(5433), 1558-1562. https://doi.org/10.1126/science.285.5433.1558