TY - JOUR
T1 - Whole-genome shotgun optical mapping of Deinococcus radiodurans
AU - Lin, Jieyi
AU - Qi, Rong
AU - Aston, Christopher
AU - Jing, Junping
AU - Anantharaman, Thomas S.
AU - Mishra, Bud
AU - White, Owen
AU - Daly, Michael J.
AU - Minton, Kenneth W.
AU - Venter, J. Craig
AU - Schwartz, David C.
PY - 1999/9/3
Y1 - 1999/9/3
N2 - A whole-genome restriction map of Deinococcus radiodurans, a radiation- resistant bacterium able to survive up to 15,000 grays of ionizing radiation, was constructed without using DNA libraries, the polymerase chain reaction, or electrophoresis. Very large, randomly sheared, genomic DNA fragments were used to construct maps from individual DNA molecules that were assembled into two circular overlapping maps (2.6 and 0.415 megabases), without gaps. A third smaller chromosome (176 kilobases)was identified and characterized. Aberrant nonlinear DNA structures that may define chromosome structure and organization, as well as intermediates in DNA repair, were directly visualized by optical mapping techniques after γ irradiation.
AB - A whole-genome restriction map of Deinococcus radiodurans, a radiation- resistant bacterium able to survive up to 15,000 grays of ionizing radiation, was constructed without using DNA libraries, the polymerase chain reaction, or electrophoresis. Very large, randomly sheared, genomic DNA fragments were used to construct maps from individual DNA molecules that were assembled into two circular overlapping maps (2.6 and 0.415 megabases), without gaps. A third smaller chromosome (176 kilobases)was identified and characterized. Aberrant nonlinear DNA structures that may define chromosome structure and organization, as well as intermediates in DNA repair, were directly visualized by optical mapping techniques after γ irradiation.
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U2 - 10.1126/science.285.5433.1558
DO - 10.1126/science.285.5433.1558
M3 - Article
C2 - 10477518
AN - SCOPUS:0033520409
SN - 0036-8075
VL - 285
SP - 1558
EP - 1562
JO - Science
JF - Science
IS - 5433
ER -