TY - JOUR
T1 - X-gal staining of electroporated sea squirt (Ciona) embryos
AU - Christiaen, Lionel
AU - Wagner, Eileen
AU - Shi, Weiyang
AU - Levine, Michael
PY - 2009
Y1 - 2009
N2 - This protocol describes the fixation, staining, and mounting of sea squirt (Ciona intestinalis) embryos and larvae that have been electroporated with a plasmid DNA containing a cis-regulatory DNA (e.g., tissue-specific enhancer) fused to the lacZ reporter gene. Green fluorescent protein (GFP) reporter genes can be directly visualized in living embryos and larvae, although fixed preparations can use aspects of this protocol.
AB - This protocol describes the fixation, staining, and mounting of sea squirt (Ciona intestinalis) embryos and larvae that have been electroporated with a plasmid DNA containing a cis-regulatory DNA (e.g., tissue-specific enhancer) fused to the lacZ reporter gene. Green fluorescent protein (GFP) reporter genes can be directly visualized in living embryos and larvae, although fixed preparations can use aspects of this protocol.
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U2 - 10.1101/pdb.prot5346
DO - 10.1101/pdb.prot5346
M3 - Article
C2 - 20150093
AN - SCOPUS:72149107101
SN - 1940-3402
VL - 4
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 12
ER -