TY - JOUR
T1 - Yeast as a tool to study Bax/mitochondrial interactions in cell death
AU - Priault, Muriel
AU - Camougrand, Nadine
AU - Kinnally, Kathleen W.
AU - Vallette, François M.
AU - Manon, Stéphen
N1 - Funding Information:
Research in the laboratory of S.M. was supported by the Centre National de la Recherche Scientifique, the Association pour la Recherche contre le Cancer, the Conseil Régional d’Aquitaine and the Université de Bordeaux 2. Research was also supported by the Ligue Nationale contre le Cancer and the Conseil Régional Bretagne-Pays de Loire to F.M.V. and by National Institute of Health Grant GM57249 to K.W.K.
PY - 2003/10
Y1 - 2003/10
N2 - The budding yeast Saccharomyces cerevisiae has proven to be a powerful tool in investigations of the molecular aspects of the events involved in apoptosis, particularly the steps implicating mitochondria. Yeast does not have obvious homologs of the proteins involved in the regulation of apoptosis, and provides a simplified model system in which the function of these proteins can be unraveled. This review focuses on the interactions of two of the major pro-apoptotic Bcl-2 family members, Bax and Bid, with mitochondria. It is shown that yeast has allowed questioning of several crucial aspects of the function of these two proteins, namely the molecular mechanisms driving their insertion into the mitochondrial outer membrane and those leading to the permeabilization to cytochrome c. More recently, signaling pathways leading to Bax-induced cell death, as well as other forms of cell death, have been identified in yeast. Both 'apoptosis-like' and autophagy-related forms of cell degradation are involved, and mitochondria play a central role in these two signaling pathways.
AB - The budding yeast Saccharomyces cerevisiae has proven to be a powerful tool in investigations of the molecular aspects of the events involved in apoptosis, particularly the steps implicating mitochondria. Yeast does not have obvious homologs of the proteins involved in the regulation of apoptosis, and provides a simplified model system in which the function of these proteins can be unraveled. This review focuses on the interactions of two of the major pro-apoptotic Bcl-2 family members, Bax and Bid, with mitochondria. It is shown that yeast has allowed questioning of several crucial aspects of the function of these two proteins, namely the molecular mechanisms driving their insertion into the mitochondrial outer membrane and those leading to the permeabilization to cytochrome c. More recently, signaling pathways leading to Bax-induced cell death, as well as other forms of cell death, have been identified in yeast. Both 'apoptosis-like' and autophagy-related forms of cell degradation are involved, and mitochondria play a central role in these two signaling pathways.
KW - AIF, apoptosis-inducing factor
KW - ANT, adenine nucleotide translocator
KW - Apoptosis
KW - Autophagy
KW - Bax
KW - Cytochrome c
KW - MAC, mitochondrial apoptosis-induced channel
KW - Mitochondrion
KW - PTP, permeability transition pore
KW - ROS, reactive oxygen species
KW - Yeast
UR - http://www.scopus.com/inward/record.url?scp=1042264219&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1042264219&partnerID=8YFLogxK
U2 - 10.1016/S1567-1356(03)00143-0
DO - 10.1016/S1567-1356(03)00143-0
M3 - Short survey
C2 - 14554193
AN - SCOPUS:1042264219
SN - 1567-1356
VL - 4
SP - 15
EP - 27
JO - FEMS Yeast Research
JF - FEMS Yeast Research
IS - 1
ER -