Yeast one-hybrid assays for gene-centered human gene regulatory network mapping

John S. Reece-Hoyes, A. Rasim Barutcu, Rachel Patton McCord, Jun Seop Jeong, Lizhi Jiang, Andrew MacWilliams, Xinping Yang, Kourosh Salehi-Ashtiani, David E. Hill, Seth Blackshaw, Heng Zhu, Job Dekker, Albertha J.M. Walhout

Research output: Contribution to journalArticle

Abstract

Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.

Original languageEnglish (US)
Pages (from-to)1050-1054
Number of pages5
JournalNature methods
Volume8
Issue number12
DOIs
StatePublished - Dec 2011

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Reece-Hoyes, J. S., Barutcu, A. R., McCord, R. P., Jeong, J. S., Jiang, L., MacWilliams, A., Yang, X., Salehi-Ashtiani, K., Hill, D. E., Blackshaw, S., Zhu, H., Dekker, J., & Walhout, A. J. M. (2011). Yeast one-hybrid assays for gene-centered human gene regulatory network mapping. Nature methods, 8(12), 1050-1054. https://doi.org/10.1038/nmeth.1764