Z-DNA conformation of N-2-acetylaminofluorene modified poly(dG-dC)·poly(dG-dC) determined by reactivity with anti cytidine antibodies and minimized potential energy calculations

Regina M. Santella, Dezider Grunberger, Suse Broyde, Brian E. Hingerty

Research output: Contribution to journalArticle

Abstract

The conformation of poly(dG-dC)·poly(dG-dC), poly(dG)·poly(dC), and calf thymus DNA modified with N-acetoxy-N-2-acetylaminofluorene (N-acetoxy-AAF) was examined by extent of reaction with anti cytidine antibodies. In contrast to modified poly(dG)·poly(dC) and DNA, modified poly(dG-dC)·poly(dG-dC) failed to react with the antibodies indicating that the base pairing in this polymer is intact. This is consistent with induction of the Z-DNA conformation in AAF modified poly(dG-dC)·poly(dG-dC). Using minimized potential energy calculations on the dCpdG-AAF dimer as a model for the modified polymer, it is shown that the proposed Z-DNA conformation is energetically stable. A model is proposed for an AAF modified tetramer, dGpdCpdGpdC, in which the AAF is external to the Z-DNA duplex.

Original languageEnglish (US)
Pages (from-to)5459-5467
Number of pages9
JournalNucleic acids research
Volume9
Issue number20
DOIs
StatePublished - Oct 24 1981

ASJC Scopus subject areas

  • Genetics

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