Zika virus specific diagnostic epitope discovery

Maite Sabalza, Cheryl A. Barber, William R. Abrams, Richard Montagna, Daniel Malamud

    Research output: Contribution to journalArticlepeer-review


    High-density peptide microarrays allow screening of more than six thousand peptides on a single standard microscopy slide. This method can be applied for drug discovery, therapeutic target identification, and developing of diagnostics. Here, we present a protocol to discover specific Zika virus (ZIKV) diagnostic peptides using a high-density peptide microarray. A human serum sample validated for ZIKV infection was incubated with a high-density peptide microarray containing the entire ZIKV protein translated into 3,423 unique 15 linear amino acid (aa) residues with a 14-aa residue overlap printed in duplicate. Staining with different secondary antibodies within the same array, we detected peptides that bind to Immunoglobulin M (IgM) and Immunoglobulin G (IgG) antibodies present in serum. These peptides were selected for further validation experiments. In this protocol, we describe the strategy followed to design, process, and analyze a high-density peptide microarray.

    Original languageEnglish (US)
    Article numbere56784
    JournalJournal of Visualized Experiments
    Issue number130
    StatePublished - Dec 12 2017


    • Diagnostic peptides
    • High-throughput screening
    • IgG
    • IgM
    • Immunoassay
    • Immunology
    • Issue 130
    • Microarray
    • Zika

    ASJC Scopus subject areas

    • General Neuroscience
    • General Chemical Engineering
    • General Biochemistry, Genetics and Molecular Biology
    • General Immunology and Microbiology


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