Zika virus specific diagnostic epitope discovery

Maite Sabalza, Cheryl A. Barber, William R. Abrams, Richard Montagna, Daniel Malamud

Research output: Contribution to journalArticlepeer-review

Abstract

High-density peptide microarrays allow screening of more than six thousand peptides on a single standard microscopy slide. This method can be applied for drug discovery, therapeutic target identification, and developing of diagnostics. Here, we present a protocol to discover specific Zika virus (ZIKV) diagnostic peptides using a high-density peptide microarray. A human serum sample validated for ZIKV infection was incubated with a high-density peptide microarray containing the entire ZIKV protein translated into 3,423 unique 15 linear amino acid (aa) residues with a 14-aa residue overlap printed in duplicate. Staining with different secondary antibodies within the same array, we detected peptides that bind to Immunoglobulin M (IgM) and Immunoglobulin G (IgG) antibodies present in serum. These peptides were selected for further validation experiments. In this protocol, we describe the strategy followed to design, process, and analyze a high-density peptide microarray.

Original languageEnglish (US)
Article numbere56784
JournalJournal of Visualized Experiments
Volume2017
Issue number130
DOIs
StatePublished - Dec 12 2017

Keywords

  • Diagnostic peptides
  • High-throughput screening
  • IgG
  • IgM
  • Immunoassay
  • Immunology
  • Issue 130
  • Microarray
  • Zika

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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