ZnS-nanocrystals/polypyrrole nanocomposite film based immunosensor

Sujeet K. Mishra; Renu Pasricha; Ashok M. Biradar; Rajesh

doi:10.1063/1.3681580

ZnS-nanocrystals/polypyrrole nanocomposite film based immunosensor

Sujeet K. Mishra, Renu Pasricha, Ashok M. Biradar, Rajesh

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Abstract

We report an electrochemically synthesized ZnS nanocrystals modified polypyrrole (PPy) nanocomposite film based immunosensor for the detection of C-reactive protein (CRP). The ZnS-PPy composite film was characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM) and electrochemical techniques. The modified film showed good biocompatibility with efficient binding to protein antibody (αCRP-Ab) molecules through ZnS nanocrystals, exhibited an attractive platform for immunosensor fabrication. The electrical and sensing properties of the polymer composite film of different thickness towards protein antigen (αCRP-Ag) were delineated. The immunosensor exhibited an impedance response to αCRP-Ag concentration in a linear range from 10 ng to 10 g mL ^-1.

Original language	English (US)
Article number	053701
Journal	Applied Physics Letters
Volume	100
Issue number	5
DOIs	https://doi.org/10.1063/1.3681580
State	Published - Jan 30 2012

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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10.1063/1.3681580

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title = "ZnS-nanocrystals/polypyrrole nanocomposite film based immunosensor",

abstract = "We report an electrochemically synthesized ZnS nanocrystals modified polypyrrole (PPy) nanocomposite film based immunosensor for the detection of C-reactive protein (CRP). The ZnS-PPy composite film was characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM) and electrochemical techniques. The modified film showed good biocompatibility with efficient binding to protein antibody (αCRP-Ab) molecules through ZnS nanocrystals, exhibited an attractive platform for immunosensor fabrication. The electrical and sensing properties of the polymer composite film of different thickness towards protein antigen (αCRP-Ag) were delineated. The immunosensor exhibited an impedance response to αCRP-Ag concentration in a linear range from 10 ng to 10 g mL -1.",

author = "Mishra, {Sujeet K.} and Renu Pasricha and Biradar, {Ashok M.} and Rajesh",

note = "Funding Information: We are grateful to Prof. R. C. Budhani, Director, National Physical Laboratory, New Delhi, India, for providing research facilities. One of the authors S. K. Mishra is thankful to CSIR for providing Senior Research Fellowship. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

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doi = "10.1063/1.3681580",

language = "English (US)",

volume = "100",

journal = "Applied Physics Letters",

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AU - Mishra, Sujeet K.

AU - Pasricha, Renu

AU - Biradar, Ashok M.

AU - Rajesh,

N1 - Funding Information: We are grateful to Prof. R. C. Budhani, Director, National Physical Laboratory, New Delhi, India, for providing research facilities. One of the authors S. K. Mishra is thankful to CSIR for providing Senior Research Fellowship. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2012/1/30

Y1 - 2012/1/30

N2 - We report an electrochemically synthesized ZnS nanocrystals modified polypyrrole (PPy) nanocomposite film based immunosensor for the detection of C-reactive protein (CRP). The ZnS-PPy composite film was characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM) and electrochemical techniques. The modified film showed good biocompatibility with efficient binding to protein antibody (αCRP-Ab) molecules through ZnS nanocrystals, exhibited an attractive platform for immunosensor fabrication. The electrical and sensing properties of the polymer composite film of different thickness towards protein antigen (αCRP-Ag) were delineated. The immunosensor exhibited an impedance response to αCRP-Ag concentration in a linear range from 10 ng to 10 g mL -1.

AB - We report an electrochemically synthesized ZnS nanocrystals modified polypyrrole (PPy) nanocomposite film based immunosensor for the detection of C-reactive protein (CRP). The ZnS-PPy composite film was characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM) and electrochemical techniques. The modified film showed good biocompatibility with efficient binding to protein antibody (αCRP-Ab) molecules through ZnS nanocrystals, exhibited an attractive platform for immunosensor fabrication. The electrical and sensing properties of the polymer composite film of different thickness towards protein antigen (αCRP-Ag) were delineated. The immunosensor exhibited an impedance response to αCRP-Ag concentration in a linear range from 10 ng to 10 g mL -1.

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JF - Applied Physics Letters

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ZnS-nanocrystals/polypyrrole nanocomposite film based immunosensor

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