We report an electrochemically synthesized ZnS nanocrystals modified polypyrrole (PPy) nanocomposite film based immunosensor for the detection of C-reactive protein (CRP). The ZnS-PPy composite film was characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM) and electrochemical techniques. The modified film showed good biocompatibility with efficient binding to protein antibody (αCRP-Ab) molecules through ZnS nanocrystals, exhibited an attractive platform for immunosensor fabrication. The electrical and sensing properties of the polymer composite film of different thickness towards protein antigen (αCRP-Ag) were delineated. The immunosensor exhibited an impedance response to αCRP-Ag concentration in a linear range from 10 ng to 10 g mL -1.
ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)